Metamorphosis of supercritical fluid chromatography: A viable tool for the analysis of polar compounds?

Recent developments in supercritical fluid chromatography (SFC) have highlighted the applicability of this technique for analysis highly polar compounds. The combination stationary phases and CO2-based mobile with an increasing presence liquid co-solvent (up to 100%) has enabled further expand application field SFC towards a variety samples such as endogenous metabolites, plant extracts, water-soluble vitamins, pesticides, sugars, peptides so on. In evolution, key role was played by addition up 5–10% water co-solvent. Moreover, higher concentrations additives, 75–100 mM some cases. These improved conditions were fundamental expanding range SFC. Overall, demonstrated its evolution into mature capable offering true alternative